Cell morphology of filamentous actinobacterium analysed by CytoSense

24.09.2020  by  Tina Silovic


Figure 1: Exemplary signal profiles of flow cytometry measurements including FSC (black), SSC (blue), green fluorescence (green) and red fluorescence (red) data.

Assessment of viable biomass of filamentous microorganisms  in industrial fermentations is challenging, while reliable monitoring tools in an at-line capacity are scarce to date. Earlier this year Vees et al., developed a robust CytoSense based method to analyze yeast cell viability and morphology in media with a high particle background. More importantly, the data produced by CytoSense provided an important insight into S. cerevisiaenews item).

In the new study by Schrinner et al., the use of Cytosense is extended to examining pellet morphology and physiology of the rebeccamycin producing filamentous actinomycete Lentzea aerocolonigenesVeiter et al., 2019.
The authors concluded that the morphological and physiological assessment via flow cytometry is an additional powerful and statistically safe method to quantify the vitality of pellets in a faster and more reliable way than before.

For more details on this exciting research, please click on the following link.