Looking inside an algae cell

05.07.2017  by  Lucyna Wlodarczyk

Images made with the CytoSub during a cruise in the North Sea.

The particles are optically magnified (16x) and captured by a 2.3 megapixel monochrome camera.

With our Imaging-In-Flow system you can get a good impression of the 3D spatial structure of the cells. The exemplary pictures made with the CytoSub reveal that all chloroplasts are located at the cell wall leaving the interior of the algae cell empty. This depth insight is achieved thanks to the narrow focal plane of imaging. As a result, only the structures that flow exactly through the focal plane are in focus leading to informative variation in sharpness.

The primary CytoSense data classification is performed with the laser excited light scattering and fluorescence profiles. The photographs give additional identification power and can be restricted to 'in focus' particles, which may be pre-selected by a new software tool available shortly.