More and more of the aquatic microworld to be monitored with the CytoSense

14.12.2017  by  Lucyna Wlodarczyk

Heterotrophic prokaryotes (HP) and heterotrophic nanoflagellates (HNF) dynamics observed by means of the CytoSense equipped with the new automated staining module. Source: Silovic et al. 2017

In this work the researchers from the Mediterranean Institute of Oceanography (MIO) demonstrate that the CytoSense can detect not only auto-fluorescing phytoplankton, but also microorganisms that do not fluoresce naturally, such as bacteria and heterotrophic nanoflagellates.

This fantastic new functionality of the CytoSense is possible thanks to the automated staining module, invented and developed in collaboration between the scientists from MIO and the CytoBuoy team. As a result, the non-fluorescent aquatic microorganisms are stained and analyzed in a controlled and automated way in the CytoSense. With this process being automatically repeated every 2 hours, the researchers were able to monitor the abundance of heterotrophic nanoflagellates (HNF) and heterotrophic prokaryotes (HP) with high frequency.

This is a great step towards a thorough understanding of the natural marine communities, in which bacteria are the main consumers and mineralisers, while the heterotrophic nanoflagellates and ciliates pose an important stage in the marine food web.

The automated staining module compatible with the CytoSense will be available soon. 

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