Looking inside a particle - CytoSense 'true imaging feature'.

03.10.2019  by  George Dubelaar

Figure 1. Diversity of Volvox colonies

With our Imaging-In-Flow system you can get a good impression of the 3D spatial structure of the cells and organisms, explained by some exemplary pictures made of Volvox colonies (Figure 1) flowing at 2 m/s through the CytoSense super wide flow cell.  The resolution is relatively high, even for very large particles so a lot of fine detail can be seen like the little eye-spots of the individual cells. These Volvox images reveal that all cells including the newly formed mini colonies are located at the circumference only, leaving the interior of the spherical colony empty. This depth insight is achieved thanks to the narrow focal plane of imaging. As a result, only the structures that flow exactly through the focal plane are in sharp focus whereas other parts are not, leading to informative character of the images. It also allows even to 'scan through' the particles because almost always many more images of the same species are observed like in this example.
CytoSense counts and analyses all particles flowing through the flowcell easily by the laser scanning, whereas only particles flowing totally or partly through the camera focal plane are photographed. This is done by realtime preselection based on the laser emission.  Of all photographed particles, their original concentration is generated by the primary CytoSense scan data classification which is performed with the laser excited light scattering and fluorescence profiles. This also groups the available photos per species immediately for efficient taxonomic analysis.