EXCITATION - EMISSION EXPERIMENT

28.11.2014  by  George Dubelaar


Which combination of lasers and fluorescence colors is optimal to achieve good identification of the various pigment types of algae?  How does this influence the detection of very small picoplankton species?

To get a better understanding of this we asked assistance of several of our customers and thanks to their help we are now running tests with 5 instruments simultaneously!  Some are single laser machines, some are double laser machines, some have 6 detector channels, some have 12.  We are testing several algal cultures with all these machines configured in different fluorescence excitation / emission combinations.

The expected results will help us to better advise our customers with regards to the configuration of their plankton flow cytometer.

Instruments kindly provided by:

  • Rijkswaterstaat, Ministry of Infrastructure and the Environment, Lelystad, NL 
  • Aarhus University, Faculty of Science and Technology, Department of Bioscience, DK
  • Universidad de Las Palmas de Gran Canaria, Departamento de Biología, ES
  • Bar Ilan University, Ramat Gan, Israel