CytoSense imaging reaches 1 Ám resolution and higher pixel density

06.11.2015  by  George Dubelaar

The CytoSense makes photos of subsets of particles. A laser scan is captured from each particle at high fluid velocity, which allows extremely fast analysis and efficient data processing and discrimination. In this way we can target/restrict the imaging process to prevent cluttering of the photo stream with redundant images of dominant species - aiming to catch the less abundant and rare particles on photo.

The image magnification is a compromise between resolution, depth of focus and image size to capture big cells and colonies. To optimize the identification power of the CytoSense photos, we now offer a larger magnification (16x), with a smaller pixel size camera (4.8 Ám). Whereas the maximum object size reduced from 780 Ám x 624 Ám to 576x360 Ám, the number of pixels per micrometer of the object doubled to 3.3 pixels per Ám.  The optical resolution of the lens system further improved to ca. 1 Ám. 

This particularly helps to identify small cells, as shown with these first test photos made of local pond water.  For reference a 20 Ám scale bar is plotted - the thin filaments have a cell width of only 1.9 Ám and are still clearly visible.