Cell morphology of filamentous actinobacterium analysed by CytoSense

24.09.2020  by  Tina Silovic

Figure 1: Exemplary signal profiles of flow cytometry measurements including FSC (black), SSC (blue), green fluorescence (green) and red fluorescence (red) data.

Assessment of viable biomass of filamentous microorganisms  in industrial fermentations is challenging, while reliable monitoring tools in an at-line capacity are scarce to date. Earlier this year Vees et al., developed a robust CytoSense based method to analyze yeast cell viability and morphology in media with a high particle background. More importantly, the data produced by CytoSense provided an important insight into S. cerevisiae  physiology and morphology which was not accessible earlier through common online and offline biomass monitoring methods (for more details check our Mayís news item).

In the new study by Schrinner et al., the use of Cytosense is extended to examining pellet morphology and physiology of the rebeccamycin producing filamentous actinomycete Lentzea aerocolonigenes. Moreover, CytoSense results were compared with the confocal laser scanning microscopy regarding the applicability of methods. Results showed excellent agreement of both methods, each considering several thousand pellets. The main difference was that with the microscope only one pellet per sample could be investigated while CytoSense considered at least 50 pellets per sample, resulting in an increased statistical reliability. The individual pellets were analysed via spatially resolved signal profiles which represent approximations of the actual pelletís cross section (Figure 1) as previously described in Veiter et al., 2019.
The authors concluded that the morphological and physiological assessment via flow cytometry is an additional powerful and statistically safe method to quantify the vitality of pellets in a faster and more reliable way than before.

For more details on this exciting research, please click on the following link.