Analysis of Prochlorococcus marina with the CytoSense

16.12.2015  by  George Dubelaar


a CytoClus 4 scatterplot of red fluorescence versus sideward scatter, using a partial-FFT algorithm (beta)

Prochlorococcus Marina

As follow-up to our news items "detecting 100 nanometer beads", "analysis of bacteria"  and "increasing the sensitivity of CytoBuoy flow cytometers" of July 2015 and November 2014,  we show sea water data, measured recently by M. Thyssen, E. Pasero, and G. Gregori from the Mediterranean Institute of Oceanography, Aix-Marseille University (personal communication 19/11/2015).  

They successfully analyzed Prochlorococcus marina in surface (1 m) water samples collected at the SOMLIT Bay of Marseille with a CytoSense, and confirmed cell counts by analysis with a FACSCalibur flow cytometer.  Here we show a CytoClus 4 scatterplot of red fluorescence versus sideward scatter, using a partial-FFT algorithm (beta) to separate signal from noise in the pulse shapes.