Medical Applications

Whether you need to analyse some of the smallest mammalian cells, such as spermatozoa, or the large aggregated and filamentous particles, like muscle fibers or stem cell clusters - the CytoSense flow cytometer will serve you well. 

Typically required particle staining is optional for counting and morphological analysis of the particles with the CytoSense flow cytometers. It is possible thanks to

  • acquisition triggered by scattered light (and not only by fluorescence as in other flow cytometers) 
  • recording of particle-unique scans 
  • targeted imaging  at 1 um resolution 

 

If staining is needed, for example to visualise metabolic activity within a cell, we offer the Autonomous Staining Module which brings the sample handling to absolute minimum, as the stained sample is automatically drawn in by the flow cytometer. Scans of a stained particles show location of the fluorescing dye in the cell. 

Fragile cell aggregates are particularly difficult to analyse with high-throughput  techniques, therefore we implemented in the CytoSense a gentle, low shear force sampling system to maintain the integrity of the delicate structures.

The wide particle size range from  0.5 um to 0.7 mm (width) and 4 mm (length) realized in the CytoSense makes this flow cytometer a suitable instrument to study also mammalian parasites, such as Giardia, Cryptosporidium and most of nematodes. 

Check how the CytoSense flow cytometer has been used in sperm quality control: 

CytoSense flow cytometer has been used as a portable sperm monitoring instrument to analyse sperm quality of the Iberian swine on farms in Spain. The scans of light scattered by a sperm cell, showing the typical long tail, allow distinction of spermatozoa from other cells and debris by shape only and without fluorescent staining. 

 

Of course staining is still possible, and might be useful for example in life/dead discrimination or in research on mitochondrial metabolic activity. The location of the fluorochrome in a cell shows up in the scan data. The picture below shows for instance where the fluorescent stain is present in the body of sperm cell.