Bioprocess Monitoring

Automated monitoring of industrial bioprocess with the results obtained within minutes and without manual sample handling?  

Yes - with the CytoSense flow cytometer!   

The CytoSense flow cytometer is a robust system that enables moreover:

  • analysis of particles in the size range from 0.3 um to 0.7 mm (width) and 4 mm (length)
  • particle imaging with high resolution of 1 um
  • autonomous staining thanks to a compatible staining module

Check how the CytoSense flow cytometers serves in bioprocess monitoring: 

  • Real-time information on morphology and metabolic activity

Particle scanning and imaging realized in the CytoSense is used to characterize the internal and external morphology of particles. The information for a statistically relevant number of particles is available already within a few minutes. Such real-time at-line monitoring is indispensable to keep optimal conditions of cultivation. In the study by Ehrgarner et al. (2016) the changes of morphology (swelling) and of metabolic activity (viability staining) of Penicillium chrysogenum spores provided information on the quality of spore inoculum. Due to the incubation time needed for staining, the results were available after around 2 hours. The flow cytometric analysis is thus much faster than the traditional CFU (colony-forming unit) determination technique and additionally makes it possible to quantify the number of dead spores.   

  • Preserve in-liquid  spatial structure of the particles during morphological analysis

If your bioprocess particles have an extensive spatial structure it is important to preserve it during the morphological analysis. Gentle sampling realized in the CytoSense maintains the integrity and shape of particles. In a study of Penicillium chrysogenum by Ehrgartner et al. (2017) this feature led to uncovering a compactness parameter for fungal pellets which was not observed with the traditional microscopy. 

  • Clog-free operation in complex medium 

Large particles contained in a complex medium do not pose a challenge for the CytoSense. The flow cytometer can fit particles with diameter up to 0.7 mm, assuring clog-free operation. Analysis by means of the CytoSense of spore germination in complex media is described for example in Ehrgartner et al. (2016)