Software

To control our instruments and to analyze the results from flow cytometry measurements we develop our own software: CytoUSB and CytoClus. These packages are designed for control of the instrument, automated measurements, data analysis and classification. They are in constant development, and can be tuned to fit your needs perfectly.

CytoUSB

CytoUSB is a specially designed program to control the Cyto-instruments. Most important abilities are:

  • Creating and doing a wide scale of different measurements.
  • Real time visualization of data.
  • Programming the instrument for full autonomous control:
    • Time scheduling.
    •  Response to external triggers.
  • Performing instrument fine tuning, calibration, and health checking.

 CytoUSB has an easy to use and modern interface, recognizes the specific instrument configuration automatically and takes care of all peripheral issues like for instance flushing or high pressure mode.

CytoClus

CytoClus is a software package especially designed and developed to process and analyze data from the CytoBuoy instruments. It's main functions include:

  • Classification and interpretation of large amounts of particle pulse data by manual gating.
  • Calculating important statistics of datasets, e.g. total red fluorescence or mean particle size.
  • Analysis of population dynamics over time, suitable for detecting important changes in cultures or the environment.
  • Interactive targeting of species for imaging a sample, enabling identification of clusters.
  • Exporting data to other formats for analysis in other third-party programs.

CytoClus is under constant development and by close interaction with the users new features and possibilities are added very frequently.

Easyclus

Easyclus© is commercially available flow cytometric data analysis software, developed by Thomas Rutten. It works especially well with CytoSense flow cytometers, and is recommended by CytoBuoy. It's working in a Matlab® environment to perform automatic clustering, autodatabasing and classification of clusters on basis of a database. Images of algae made during the flowcytometric analysis are used to validate the results. Typical bioindicators describe the ecological status of the water.

Easyclus© is based on years of experience with handling large amounts of flow cytometric monitoring data. A lot of research, further improvement and development of new algorithms have been acquired to process multivariate flow cytometric datasets automatically and efficiently.

Portable lab
CytoClus interface
Image analysis in CytoClus

CytoUSB generates a scatterplot in real time during measurement, enabling early identification and confirmation of the sample.

An overview of EasyClus' functionality