particle imaging
Flow cytometry and simultaneous imaging of target cells, called 'flow cytometric imaging',
is a powerful procedure for phytoplankton analysis: it combines the speed, precision and
objectivity of flow cytometry with real images.
CytoSense does not take random images. The random recording of full photos is too slow
for flow cytometric count rates and would be impractible in terms of data processing, file
handling and bandwith sensitive applications using telemetry or long cables (submarine
work etc.).
The CytoSense imaging-in-flow therefore works with 'targetting imaging' which means that
instead of taking random pictures, the imaging may be targetted to specific particle types
of interest. Directly after a particle has intersected the analysing laser beam of the flow
cytometer a powerful realtime calculation proces starts up to analyze the fully scanned
optical cytometric signature (silico-image) and decide whether this particle fits the
preselected target group description. Within a few milliseconds the camera can be
triggered to make the picture. In this way, the full species/groups discrimination power of
the CytoSense particle scan data can be used seemlessly for the preselection of the
target groups which is a unique and very efficient combination of imaging and high
throughput particle analysis.
The targetted images can be used for direct identification of the selection sets (libraries)
of species resulting from the regular CytoSense data analysis.
PDF brochures for download:
image-in-flow
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